DNASE1L3 ELISA Kit

Produktnummer ABIN2043325

Maus DNASE1L3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus DNASE1L3.

Kurzübersicht für DNASE1L3 ELISA Kit (ABIN2043325)

Target: DNASE1L3 (Deoxyribonuclease I-Like 3 (DNASE1L3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails DNASE1L3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.058 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Deoxyribonuclease I Like Protein 3 (DNASE1L3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxyribonuclease I Like Protein 3 (DNASE1L3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deoxyribonuclease I Like Protein 3 (DNASE1L3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deoxyribonuclease I Like Protein 3 (DNASE1L3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Deoxyribonuclease I Like Protein 3 (DNASE1L3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DNASE1L3

Target: DNASE1L3 (Deoxyribonuclease I-Like 3 (DNASE1L3))

Andere Bezeichnung: Deoxyribonuclease I Like Protein 3 (DNASE1L3)