beta 2 Defensin ELISA Kit

Produktnummer ABIN2043311

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Rind (Kuh) beta 2 Defensin.

Kurzübersicht für beta 2 Defensin ELISA Kit (ABIN2043311)

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Produktdetails beta 2 Defensin ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Sensitivität: < 22.6 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Defensin Beta 2 (DEFb2) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 2 (DEFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 2 (DEFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 2 (DEFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 2 (DEFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu beta 2 Defensin

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Andere Bezeichnung: Defensin beta 2 (DEFb2)