DEFB126 ELISA Kit

Produktnummer ABIN2043307

Human DEFB126 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human DEFB126.

Kurzübersicht für DEFB126 ELISA Kit (ABIN2043307)

Target: DEFB126 (Defensin, beta 126 (DEFB126))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 pg/mL

Applikation: ELISA

Produktdetails DEFB126 ELISA Kit

Untere Nachweisgrenze: 7.81 pg/mL

Sensitivität: < 3.4 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Defensin Beta 126 (DEFb126) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 126 (DEFb126). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 126 (DEFb126). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 126 (DEFb126), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 126 (DEFb126) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DEFB126

Target: DEFB126 (Defensin, beta 126 (DEFB126))

Andere Bezeichnung: Defensin beta 126 (DEFb126)