DEFB124 ELISA Kit

Produktnummer ABIN2043305

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human DEFB124.

Kurzübersicht für DEFB124 ELISA Kit (ABIN2043305)

Target: DEFB124 (Defensin, beta 124 (DEFB124))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails DEFB124 ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 6.4 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Defensin Beta 124 (DEFb124) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 124 (DEFb124). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 124 (DEFb124). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 124 (DEFb124), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 124 (DEFb124) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DEFB124

Target: DEFB124 (Defensin, beta 124 (DEFB124))

Andere Bezeichnung: Defensin beta 124 (DEFb124)