DEFB103A ELISA Kit

Produktnummer ABIN2043301

Produktdetails DEFB103A ELISA Kit

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 12.7 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Defensin Beta 103B (DEFB103B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103B (DEFB103B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103B (DEFB103B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103B (DEFB103B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 103B (DEFB103B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DEFB103A

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Andere Bezeichnung: Defensin beta 103B (DEFB103B) (DEFB103A Produkte)

Pathways: Production of Molecular Mediator of Immune Response