DEFB103A ELISA Kit

Produktnummer ABIN2043299

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Rind (Kuh) DEFB103A.

Kurzübersicht für DEFB103A ELISA Kit (ABIN2043299)

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails DEFB103A ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 12.5 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Defensin Beta 103A (DEFb103A) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103A (DEFb103A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103A (DEFb103A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103A (DEFb103A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 103A (DEFb103A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DEFB103A

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Andere Bezeichnung: Defensin beta 103A (DEFb103A)

Pathways: Production of Molecular Mediator of Immune Response