beta Defensin 1 ELISA Kit

Produktnummer ABIN2043297

Dieses Rind (Kuh) beta Defensin 1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Rind (Kuh) beta Defensin 1 zu quantifizieren.

Kurzübersicht für beta Defensin 1 ELISA Kit (ABIN2043297)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails beta Defensin 1 ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 35 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Defensin Beta 1 (DEFb1) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 1 (DEFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 1 (DEFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 1 (DEFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 1 (DEFb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Andere Bezeichnung: Defensin beta 1 (DEFb1)