CLOCK ELISA Kit

Produktnummer ABIN2043169

Human CLOCK ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human CLOCK.

Kurzübersicht für CLOCK ELISA Kit (ABIN2043169)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails CLOCK ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.109 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Clock Homolog (CLOCK) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clock Homolog (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Clock Homolog (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clock Homolog (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Clock Homolog (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Andere Bezeichnung: Clock Homolog (CLOCK)

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism