Claudin 9 ELISA Kit

Produktnummer ABIN2043167

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Claudin 9.

Kurzübersicht für Claudin 9 ELISA Kit (ABIN2043167)

Target: Claudin 9 (CLDN9)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails Claudin 9 ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 33 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Claudin 9 (CLDN9) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 9 (CLDN9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 9 (CLDN9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 9 (CLDN9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Claudin 9 (CLDN9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Claudin 9

Target: Claudin 9 (CLDN9)

Pathways: Cell-Cell Junction Organization, Hepatitis C