Claudin 5 ELISA Kit

Produktnummer ABIN2043163

Maus Claudin 5 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Claudin 5.

Kurzübersicht für Claudin 5 ELISA Kit (ABIN2043163)

Target: Claudin 5 (CLDN5)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails Claudin 5 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.116 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Claudin 5 (CLDN5) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 5 (CLDN5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 5 (CLDN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 5 (CLDN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Claudin 5 (CLDN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Claudin 5

Target: Claudin 5 (CLDN5)

Pathways: Hepatitis C