Claudin 16 ELISA Kit

Produktnummer ABIN2043161

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte Claudin 16.

Kurzübersicht für Claudin 16 ELISA Kit (ABIN2043161)

Target: Claudin 16 (CLDN16)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails Claudin 16 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.53 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Claudin 16 (CLDN16) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 16 (CLDN16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 16 (CLDN16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 16 (CLDN16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Claudin 16 (CLDN16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Claudin 16

Target: Claudin 16 (CLDN16)

Pathways: Hepatitis C