CS ELISA Kit

Produktnummer ABIN2043153

Dieses Human CS ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CS zu quantifizieren.

Kurzübersicht für CS ELISA Kit (ABIN2043153)

Target: CS (Citrate Synthase (CS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25-400 ng/mL

Applikation: ELISA

Produktdetails CS ELISA Kit

Untere Nachweisgrenze: 6.25 ng/mL

Sensitivität: < 2.39 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Citrate Synthase (CS) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Citrate Synthase (CS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Citrate Synthase (CS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Citrate Synthase (CS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Citrate Synthase (CS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CS

Target: CS (Citrate Synthase (CS))