C1orf85 ELISA Kit

Produktnummer ABIN2043151

Maus C1orf85 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus C1orf85.

Kurzübersicht für C1orf85 ELISA Kit (ABIN2043151)

Target: C1orf85 (C1ORF85) (Chromosome 1 Open Reading Frame 85 (C1ORF85))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails C1orf85 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.123 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Chromosome 1 Open Reading Frame 85 (C1orf85) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromosome 1 Open Reading Frame 85 (C1orf85). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromosome 1 Open Reading Frame 85 (C1orf85). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromosome 1 Open Reading Frame 85 (C1orf85), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chromosome 1 Open Reading Frame 85 (C1orf85) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu C1orf85

Target: C1orf85 (C1ORF85) (Chromosome 1 Open Reading Frame 85 (C1ORF85))