CGB ELISA Kit

Produktnummer ABIN2043149

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human CGB.

Kurzübersicht für CGB ELISA Kit (ABIN2043149)

Target: CGB (Chorionic Gonadotropin, beta Polypeptide (CGB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25-400 ng/mL

Applikation: ELISA

Produktdetails CGB ELISA Kit

Untere Nachweisgrenze: 6.25 ng/mL

Sensitivität: < 2.75 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Chorionic Gonadotropin Beta Polypeptide (CGb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chorionic Gonadotropin Beta Polypeptide (CGb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CGB

Target: CGB (Chorionic Gonadotropin, beta Polypeptide (CGB))

Andere Bezeichnung: Chorionic Gonadotropin beta Polypeptide (CGb)