CHRNB1 ELISA Kit

Produktnummer ABIN2043147

Dieses Ratte CHRNB1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte CHRNB1 zu quantifizieren.

Kurzübersicht für CHRNB1 ELISA Kit (ABIN2043147)

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails CHRNB1 ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 31 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CHRNB1

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Andere Bezeichnung: Cholinergic Receptor, Nicotinic, beta 1 (CHRNb1)

Pathways: Synaptic Membrane