Chitotriosidase 1 ELISA Kit

Produktnummer ABIN2043135

Produktdetails Chitotriosidase 1 ELISA Kit

Target: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.134 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Chitinase 1 (CHIT1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase 1 (CHIT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chitinase 1 (CHIT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chitinase 1 (CHIT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Chitinase 1 (CHIT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Chitotriosidase 1

Target: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Andere Bezeichnung: Chitinase 1 (CHIT1) (CHIT1 Produkte)

Synonyme: CHI3 ELISA Kit, CHIT ELISA Kit, CHITD ELISA Kit, CHIT1 ELISA Kit, chio ELISA Kit, 2300002L19Rik ELISA Kit, chitinase 1 ELISA Kit, chitinase 1 (chitotriosidase) ELISA Kit, CHIT1 ELISA Kit, chit1 ELISA Kit, Chit1 ELISA Kit