CD5L ELISA Kit

Produktnummer ABIN2043103

Human CD5L ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human CD5L.

Kurzübersicht für CD5L ELISA Kit (ABIN2043103)

Target: CD5L (CD5 Molecule-Like (CD5L))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Produktdetails CD5L ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Sensitivität: < 28.6 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: CD5 Antigen Like Protein (CD5L) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD5 Antigen Like Protein (CD5L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD5 Antigen Like Protein (CD5L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD5 Antigen Like Protein (CD5L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of CD5 Antigen Like Protein (CD5L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CD5L

Target: CD5L (CD5 Molecule-Like (CD5L))

Andere Bezeichnung: CD5 Antigen Like Protein (CD5L)