CTSA ELISA Kit

Produktnummer ABIN2043087

Dieses Human CTSA ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CTSA zu quantifizieren.

Kurzübersicht für CTSA ELISA Kit (ABIN2043087)

Target: CTSA (Cathepsin A (CTSA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails CTSA ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Marke: BioAssay™

Sensitivität: < 0.103 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Cathepsin A (CTSA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin A (CTSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin A (CTSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin A (CTSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cathepsin A (CTSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CTSA

Target: CTSA (Cathepsin A (CTSA))