Cathelicidin ELISA Kit

Produktnummer ABIN2043085

Maus Cathelicidin ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus Cathelicidin.

Kurzübersicht für Cathelicidin ELISA Kit (ABIN2043085)

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails Cathelicidin ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.129 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Cathelicidin Antimicrobial Peptide (CAMP) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathelicidin Antimicrobial Peptide (CAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Cathelicidin

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Pathways: Cellular Response to Molecule of Bacterial Origin