FLASH ELISA Kit

Produktnummer ABIN2043079

Ratte FLASH ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte FLASH.

Kurzübersicht für FLASH ELISA Kit (ABIN2043079)

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails FLASH ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.136 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Caspase 8 Associated Protein 2 (CASP8AP2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 Associated Protein 2 (CASP8AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 Associated Protein 2 (CASP8AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8 Associated Protein 2 (CASP8AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Caspase 8 Associated Protein 2 (CASP8AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu FLASH

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Andere Bezeichnung: Caspase 8 Associated Protein 2 (CASP8AP2)

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity