CAMKK2 ELISA Kit

Produktnummer ABIN2043051

Das Human CAMKK2 ELISA Kit (ABIN2043051) ist ein Colorimetric ELISA Kit zur Detektion von Human CAMKK2.

Kurzübersicht für CAMKK2 ELISA Kit (ABIN2043051)

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails CAMKK2 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.114 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CAMKK2

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Andere Bezeichnung: Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2)

Pathways: AMPK Signaling