CASR ELISA Kit

Produktnummer ABIN2043047

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus CASR.

Kurzübersicht für CASR ELISA Kit (ABIN2043047)

Target: CASR (Calcium-Sensing Receptor (CASR))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails CASR ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.057 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Calcium Sensing Receptor (CASR) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Sensing Receptor (CASR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Sensing Receptor (CASR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Sensing Receptor (CASR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Calcium Sensing Receptor (CASR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CASR

Target: CASR (Calcium-Sensing Receptor (CASR))

Andere Bezeichnung: Calcium Sensing Receptor (CASR)

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis