BMP8A ELISA Kit

Produktnummer ABIN2043013

Ratte BMP8A ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte BMP8A.

Kurzübersicht für BMP8A ELISA Kit (ABIN2043013)

Target: BMP8A (Bone Morphogenetic Protein 8A (BMP8A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails BMP8A ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.116 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Bone Morphogenetic Protein 8A (BMP8A) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 8A (BMP8A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bone Morphogenetic Protein 8A (BMP8A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu BMP8A

Target: BMP8A (Bone Morphogenetic Protein 8A (BMP8A))

Pathways: Negative Regulation of Hormone Secretion