BHMT ELISA Kit

Produktnummer ABIN2043009

Dieses Human BHMT ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human BHMT zu quantifizieren.

Kurzübersicht für BHMT ELISA Kit (ABIN2043009)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails BHMT ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.124 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Betaine Homocysteine Methyltransferase (BHMT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Andere Bezeichnung: Betaine Homocysteine Methyltransferase (BHMT)

Pathways: Methionine Biosynthetic Process