AVPR2 ELISA Kit

Produktnummer ABIN2042939

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus AVPR2.

Kurzübersicht für AVPR2 ELISA Kit (ABIN2042939)

Target: AVPR2 (Arginine Vasopressin Receptor 2 (AVPR2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails AVPR2 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.118 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Arginine Vasopressin Receptor 2 (AVPR2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginine Vasopressin Receptor 2 (AVPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arginine Vasopressin Receptor 2 (AVPR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu AVPR2

Target: AVPR2 (Arginine Vasopressin Receptor 2 (AVPR2))

Pathways: cAMP Metabolic Process