ALOX12 ELISA Kit

Produktnummer ABIN2042935

Human ALOX12 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ALOX12.

Kurzübersicht für ALOX12 ELISA Kit (ABIN2042935)

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails ALOX12 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Marke: BioAssay™

Sensitivität: < 0.116 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Arachidonate-12-Lipoxygenase (ALOX12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ALOX12

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Andere Bezeichnung: Arachidonate-12-Lipoxygenase (ALOX12)

Pathways: Positive Regulation of Endopeptidase Activity