APOA1BP ELISA Kit

Produktnummer ABIN2042921

Produktdetails APOA1BP ELISA Kit

Target: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Apolipoprotein A1 Binding Protein (APOA1BP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 Binding Protein (APOA1BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A1 Binding Protein (APOA1BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu APOA1BP

Target: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Andere Bezeichnung: Apolipoprotein A1 Binding Protein (APOA1BP) (APOA1BP Produkte)

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