Anti-Sperm Antibody (AsAb) ELISA Kit

Produktnummer ABIN2042911

Human Anti-Sperm Antibody ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Anti-Sperm Antibody.

Kurzübersicht für Anti-Sperm Antibody (AsAb) ELISA Kit (ABIN2042911)

Target: Anti-Sperm Antibody (AsAb)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.59 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Anti-Sperm Antibody (AsAb) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Sperm Antibody (AsAb) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Sperm Antibody (AsAb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Antigendetails

Target: Anti-Sperm Antibody (AsAb)

Substanzklasse: Antibody