Anti-Myeloperoxidase Antibody (MPO pANCA) ELISA Kit

Produktnummer ABIN2042907

Das Human Anti-Myeloperoxidase Antibody ELISA Kit (ABIN2042907) ist ein Colorimetric ELISA Kit zur Detektion von Human Anti-Myeloperoxidase Antibody.

Kurzübersicht für Anti-Myeloperoxidase Antibody (MPO pANCA) ELISA Kit (ABIN2042907)

Target: Anti-Myeloperoxidase Antibody (MPO pANCA)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 ng/mL

Applikation: ELISA

Produktdetails

Untere Nachweisgrenze: 3.12 ng/mL

Sensitivität: < 1.12 ng/mL

Bestandteile: A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Assay Diluent A, 1x10 mL
F: TMB Substrate, 1x0 mL
G: Stop Solution, 1x0 mL
H: Wash Buffer, 30X, 1x20 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: The Human Anti-Myeloperoxidase Antibody (Anti-MPO) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-MPO in human serum, plasma and other biological fluids.The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-MPO will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Antigendetails

Target: Anti-Myeloperoxidase Antibody (MPO pANCA)

Substanzklasse: Antibody