Anti-Heparin/Platelet Factor 4 Antibodies ELISA Kit

Produktnummer ABIN2042891

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human .

Kurzübersicht für Anti-Heparin/Platelet Factor 4 Antibodies ELISA Kit (ABIN2042891)

Target: Anti-Heparin/Platelet Factor 4 Antibodies

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.64 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Heparin/Platelet Factor 4 Antibodies (Anti-HPF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Antigendetails

Target: Anti-Heparin/Platelet Factor 4 Antibodies

Substanzklasse: Antibody