Annexin a1 ELISA Kit

Produktnummer ABIN2042877

Dieses Kaninchen Annexin a1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Kaninchen Annexin a1 zu quantifizieren.

Kurzübersicht für Annexin a1 ELISA Kit (ABIN2042877)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reaktivität: Kaninchen

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails Annexin a1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.131 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Annexin A1 (ANXA1) BioAssay-ELISA Kit (Rabbit) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A1 (ANXA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Annexin A1 (ANXA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Pathways: Hormone Transport