C19ORF80 ELISA Kit

Produktnummer ABIN2042857

Human C19ORF80 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human C19ORF80.

Kurzübersicht für C19ORF80 ELISA Kit (ABIN2042857)

Target: C19ORF80 (Chromosome 19 Open Reading Frame 80 (C19ORF80))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails C19ORF80 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.054 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Angiopoietin Like Protein 8 (ANGPTL8) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 8 (ANGPTL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu C19ORF80

Target: C19ORF80 (Chromosome 19 Open Reading Frame 80 (C19ORF80))

Andere Bezeichnung: Angiopoietin Like Protein 8 (ANGPTL8)