AMACR ELISA Kit

Produktnummer ABIN2042825

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human AMACR.

Kurzübersicht für AMACR ELISA Kit (ABIN2042825)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails AMACR ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Alpha-Methylacyl Coenzyme A Racemase (aMACR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Methylacyl Coenzyme A Racemase (aMACR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-Methylacyl Coenzyme A Racemase (aMACR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Andere Bezeichnung: alpha-Methylacyl Coenzyme A Racemase (aMACR)

Pathways: Monocarboxylic Acid Catabolic Process