alpha Fetoprotein ELISA Kit

Produktnummer ABIN2042821

Affe alpha Fetoprotein ELISA-Kit Colorimetric-Assay zur Quantifizierung von Affe alpha Fetoprotein.

Kurzübersicht für alpha Fetoprotein ELISA Kit (ABIN2042821)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Affe

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails alpha Fetoprotein ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.118 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Alpha-Fetoprotein (aFP) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-Fetoprotein (aFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Pathways: C21-Steroid Hormone Metabolic Process