AZGP1 ELISA Kit

Produktnummer ABIN2042815

Maus AZGP1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus AZGP1.

Kurzübersicht für AZGP1 ELISA Kit (ABIN2042815)

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.25-80 ng/mL

Applikation: ELISA

Produktdetails AZGP1 ELISA Kit

Untere Nachweisgrenze: 1.25 ng/mL

Sensitivität: < 0.49 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu AZGP1

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Andere Bezeichnung: alpha-2-Glycoprotein 1, Zinc Binding (aZGP1)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process