ARL1 ELISA Kit

Produktnummer ABIN2042809

Human ARL1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ARL1.

Kurzübersicht für ARL1 ELISA Kit (ABIN2042809)

Target: ARL1 (Aldose Reductase Like Protein 1 (ARL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails ARL1 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 12.9 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Aldose Reductase Like Protein 1 (ARL1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldose Reductase Like Protein 1 (ARL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldose Reductase Like Protein 1 (ARL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldose Reductase Like Protein 1 (ARL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Aldose Reductase Like Protein 1 (ARL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ARL1

Target: ARL1 (Aldose Reductase Like Protein 1 (ARL1))