ADH1 ELISA Kit

Produktnummer ABIN2042801

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus ADH1.

Kurzübersicht für ADH1 ELISA Kit (ABIN2042801)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 ng/mL

Applikation: ELISA

Produktdetails ADH1 ELISA Kit

Untere Nachweisgrenze: 3.12 ng/mL

Sensitivität: < 1.32 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Alcohol Dehydrogenase 1 (ADH1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 1 (ADH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 1 (ADH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 1 (ADH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alcohol Dehydrogenase 1 (ADH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Andere Bezeichnung: Alcohol Dehydrogenase 1 (ADH1)