ADCY5 ELISA Kit

Produktnummer ABIN2042779

Human ADCY5 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ADCY5.

Kurzübersicht für ADCY5 ELISA Kit (ABIN2042779)

Target: ADCY5 (Adenylate Cyclase 5 (ADCY5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails ADCY5 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Marke: BioAssay™

Sensitivität: < 0.124 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Adenylate Cyclase 5 (ADCY5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 5 (ADCY5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 5 (ADCY5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 5 (ADCY5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 5 (ADCY5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ADCY5

Target: ADCY5 (Adenylate Cyclase 5 (ADCY5))

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma