ADCY10 ELISA Kit

Produktnummer ABIN2042777

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte ADCY10.

Kurzübersicht für ADCY10 ELISA Kit (ABIN2042777)

Target: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails ADCY10 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.125 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Adenylate Cyclase 10, Soluble (ADCY10) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 10, Soluble (ADCY10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 10, Soluble (ADCY10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ADCY10

Target: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Andere Bezeichnung: Adenylate Cyclase 10, Soluble (ADCY10)

Pathways: cAMP Metabolic Process