Arc ELISA Kit

Produktnummer ABIN2042773

Ratte Arc ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte Arc.

Kurzübersicht für Arc ELISA Kit (ABIN2042773)

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails Arc ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.051 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Activity Regulated Cytoskeleton Associated Protein (ARC) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activity Regulated Cytoskeleton Associated Protein (ARC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activity Regulated Cytoskeleton Associated Protein (ARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activity Regulated Cytoskeleton Associated Protein (ARC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Activity Regulated Cytoskeleton Associated Protein (ARC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Arc

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Andere Bezeichnung: Activity Regulated Cytoskeleton Associated Protein (ARC)