ACTG2 ELISA Kit

Produktnummer ABIN2042761

Human ACTG2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ACTG2.

Kurzübersicht für ACTG2 ELISA Kit (ABIN2042761)

Target: ACTG2 (Actin, gamma 2, Smooth Muscle, Enteric (ACTG2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails ACTG2 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.053 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Actin Gamma 2, Smooth Muscle (ACTg2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Gamma 2, Smooth Muscle (ACTg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Actin Gamma 2, Smooth Muscle (ACTg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ACTG2

Target: ACTG2 (Actin, gamma 2, Smooth Muscle, Enteric (ACTG2))

Andere Bezeichnung: Actin gamma 2, Smooth Muscle (ACTg2)