ACAT2 ELISA Kit

Produktnummer ABIN2042751

Dieses Maus ACAT2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus ACAT2 zu quantifizieren.

Kurzübersicht für ACAT2 ELISA Kit (ABIN2042751)

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails ACAT2 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.047 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 2 (ACAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ACAT2

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Andere Bezeichnung: Acetyl Coenzyme A Acetyltransferase 2 (ACAT2)