ADAM33 ELISA Kit

Produktnummer ABIN2042743

Human ADAM33 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ADAM33.

Kurzübersicht für ADAM33 ELISA Kit (ABIN2042743)

Target: ADAM33 (ADAM Metallopeptidase Domain 33 (ADAM33))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails ADAM33 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.060 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: A Disintegrin And Metalloprotease 33 (ADAM33) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 33 (ADAM33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of A Disintegrin And Metalloprotease 33 (ADAM33) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ADAM33

Target: ADAM33 (ADAM Metallopeptidase Domain 33 (ADAM33))

Andere Bezeichnung: A Disintegrin And Metalloprotease 33 (ADAM33)