HSD11B1L ELISA Kit

Produktnummer ABIN2042731

Human HSD11B1L ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human HSD11B1L.

Kurzübersicht für HSD11B1L ELISA Kit (ABIN2042731)

Target: HSD11B1L (Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails HSD11B1L ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.065 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HSD11B1L

Target: HSD11B1L (Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))

Andere Bezeichnung: 11-beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3)