VEGF ELISA Kit

Produktnummer ABIN2042721

Dieses Ratte VEGF ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte VEGF zu quantifizieren.

Kurzübersicht für VEGF ELISA Kit (ABIN2042721)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Produktdetails VEGF ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile: 96-well plate pre-coated with anti-VEGF, Rat
Lyophilized recombinant VEGF, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Rat VEGF in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias.VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25kD single chain as well as a 50kD dimer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Andere Bezeichnung: VEGF

Gen-ID: 83785

UniProt: B5DEK7