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VCAM1 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus VCAM1.
Produktnummer ABIN2042717

Kurzübersicht für VCAM1 ELISA Kit (ABIN2042717)

Target

Alle VCAM1 ELISA Kits anzeigen
VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reaktivität

  • 14
  • 10
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

6-10000 pg/mL

Applikation

ELISA
  • Untere Nachweisgrenze

    6 pg/mL

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 5 pg/mL

    Bestandteile

    96-well plate pre-coated with anti-VCAM-1, Mouse
    Lyophilized recombinant VCAM-1, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plattentyp

    Pre-coated

    Protokoll

    Sandwich ELISA quantitative detection kit for Mouse VCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9kD. As a result of glycosylation, the molecular mass of 120kD is revealed by SDS-PAGE.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C/-20 °C
  • Target Alle VCAM1 ELISA Kits anzeigen

    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

    Andere Bezeichnung

    VCAM-1

    Gen-ID

    22329

    UniProt

    P29533

    Pathways

    Carbohydrate Homeostasis
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