VCAM1 ELISA Kit
Kurzübersicht für VCAM1 ELISA Kit (ABIN2042717)
Target
Alle VCAM1 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
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Untere Nachweisgrenze
- 6 pg/mL
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Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivität
- < 5 pg/mL
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Bestandteile
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96-well plate pre-coated with anti-VCAM-1, Mouse
Lyophilized recombinant VCAM-1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plattentyp
- Pre-coated
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Protokoll
- Sandwich ELISA quantitative detection kit for Mouse VCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9kD. As a result of glycosylation, the molecular mass of 120kD is revealed by SDS-PAGE.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C/-20 °C
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- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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Andere Bezeichnung
- VCAM-1
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Gen-ID
- 22329
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UniProt
- P29533
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Pathways
- Carbohydrate Homeostasis
Target Alle VCAM1 ELISA Kits anzeigen
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