P-Selectin ELISA Kit

Produktnummer ABIN2042668

Maus P-Selectin ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus P-Selectin.

Kurzübersicht für P-Selectin ELISA Kit (ABIN2042668)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Produktdetails P-Selectin ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-sP-Selectin, Mouse
Lyophilized recombinant sP-Selectin, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse P-Selectin in cell culture supernates, serum and plasma (citrate).P-selectin, also called GMP-140, CD62, or selectin P, is a 140kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes.1 It is stored in secretory granules and expressed at the plasma membrane after cell activation.2 It is known to play an important role in atherosclerosis.3 The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).4 The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1kD. As a result of glycosylation, the molecular mass of 190-200kD is revealed by SDS-PAGE.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Andere Bezeichnung: sP-Selectin

Gen-ID: 20344

UniProt: Q01102