MMP13 ELISA Kit

Produktnummer ABIN2042611

Human MMP13 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human MMP13.

Kurzübersicht für MMP13 ELISA Kit (ABIN2042611)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 8-5000 pg/mL

Applikation: ELISA

Produktdetails MMP13 ELISA Kit

Untere Nachweisgrenze: 8 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-MMP13, Human
Lyophilized recombinant MMP13, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human MMP13 in cell culture supernates, serum and plasma (heparin).Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene.[1][2]Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. [3] The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Andere Bezeichnung: MMP13

Gen-ID: 4322

UniProt: A8K846