Laminin ELISA Kit

Produktnummer ABIN2042573

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Laminin.

Kurzübersicht für Laminin ELISA Kit (ABIN2042573)

Target: Laminin (LN)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-10000 pg/mL

Applikation: ELISA

Produktdetails Laminin ELISA Kit

Untere Nachweisgrenze: 6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile: A: Microtiter Plate, 1x96wells. Pre-coated with anti-human Laminin antibody
B: Laminin standard, 2x10 ng
B: Anti-human Laminin (Biotin), 1x130 µL (1:100 dilution)
C: Avidin-Biotin-Peroxidase Complex (ABC), 1x130 µL (1:100 dilution)
: Sample Diluent Buffer, 1x30 mL
: Antibody Diluent Buffer, 1x10 mL
: ABC Diluent Buffer, 1x10 mL
: TMB Color Developing Agent, 1x10 mL
: TMB Stop Solution, 2N H2SO4, 1x10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human Laminin in cell culture supernates, serum and plasma (heparin, EDTA).Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3.3 The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Laminin

Target: Laminin (LN)

Andere Bezeichnung: Laminin

Gen-ID: 3909

UniProt: B0YJ33