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Fibronectin ELISA Kit

Maus Fibronectin ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Fibronectin.
Produktnummer ABIN2042467
1.121,25 €
Zzgl. Versandkosten 20,00 € und MwSt
96 tests
Lieferung nach: Deutschland
Lieferung in 20 bis 26 Werktagen

Kurzübersicht für Fibronectin ELISA Kit (ABIN2042467)

Target

Alle Fibronectin ELISA Kits anzeigen
Fibronectin

Reaktivität

  • 10
  • 10
  • 7
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

6-10000 pg/mL

Applikation

ELISA
  • Untere Nachweisgrenze

    6 pg/mL

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 15 pg/mL

    Bestandteile

    96-well plate pre-coated with anti-Fibronectin, Mouse
    Lyophilized recombinant Fibronectin, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plattentyp

    Pre-coated

    Protokoll

    Sandwich ELISA quantitative detection kit for Mouse Fibronectin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides.1 These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris.2 Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.3

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C/-20 °C
  • Target Alle Fibronectin ELISA Kits anzeigen

    Fibronectin

    Gen-ID

    16412

    UniProt

    P09055
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